Composite

Part:BBa_K2201320:Design

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-09-23)


functional GFP-streptavidin fusion protein with medium gly-gly-ser linker and T7-promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 825
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 867
    Illegal AgeI site found at 918
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 682


Design Notes

The strain has to encode a T7-polymerase.


Source

K2201220 and K525998

References